Regular Voluntary Exercise Potentiates Interleukin-1β and Interleukin-18 Secretion by Increasing Caspase-1 Expression in Murine Macrophages

Table 1

Primers and probes used in the real-time PCR.

Protein	Gene	Probe	Forward primer sequence	Reverse primer sequence
F4/80	Emr1	#25	5′-TTA TTG TAC GTG CAA CTC AGG ACT-3′	5′-TGG AGC ACT CAT CCA CAT CT-3′
TLR4	Tlr4	#2	5′-GGA CTC TGA TCA TGG CAC TG-3′	5′-CTG ATC CAT GCA TTG GTA GGT-3′
IL-1β	Il1b	#78	5′-TGT AAT GAA AGA CGG CAC ACC-3′	5′-TCT TCT TTG GGT ATT GCT TGG-3′
IL-18	Il18	#67	5′-ACA GGC CTG ACA TCT TCT GC-3′	5′-CCT TGA AGT TGA CGC AAG AGT-3′
TNF-α	Tnf	#49	5′-TCT TCT CAT TCC TGC TTG TGG-3′	5′-GGT CTG GGC CAT AGA ACT GA-3′
IL-10	Il10	#21	5′-ACT GCA CCC ACT TCC CAG T-3′	5′-TGT CCA GCT GGT CCT TTG TT-3′
NLRP3	Nlrp3	#64	5′-GCT GTG TGA GGC ACT CCA G-3′	5′-GGA GAT GTC GAA GCA GCA TT-3′
Caspase-1	Casp1	#27	5′-CGT GAA AGT GAA AGA AAA TCT CAC-3′	5′-GTA CTG TCA GAA GTC TTG TGC TCT G-3′
18S rRNA	Rn18s	#55	5′-GGA GAA AAT CTG GCA CCA CAC CTT-3′	5′-CCT TAA TGT CAC GCA CGA TTT CCC-3′