Mediators of Inflammation / 2017 / Article / Fig 2

Research Article

Modulation of Dendritic Cell Apoptosis and CD8+ Cytotoxicity by Histamine: Role of Protein Kinase C

Figure 2

Histamine modulates presentation via the vacuolar pathway. (a), (i) A representative dot plot of DC region used for the analysis is shown. (a), (ii) The mean percentage of OVA257-264-H2Kb+ DC is significantly higher for HIS-stimulated DC. (b) We show the colocalization at the membrane of DC of class I molecules and the generated OVA epitope. (c) DC were incubated with Epo or Baf for 90 min at 37°C and then treated or not with HIS (0.1 μM). After 20 min, cells were incubated in the presence of OVA-FITC (100 μg/ml). Finally, after 2 h of culture, DC were washed and stained with anti-mouse SIINFEKL-H2Kb for 20 min at 4°C. Bars represent the % mean of OVA257–264-H2Kb+ cells ± SE of 8 independent experiments for DC inhibited with Epo (i) and Baf (ii) (, ANOVA and Tukey’s test).

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