Toll-Like Receptor 9 Promotes Survival in SERCA2a KO Heart Failure Mice

Figure 3

Increased cardiac and systemic inflammation in both HF groups, but no difference between these groups. (a) Representative images of MAC-2-stained cardiac sections. The size marker is 50 μM. (b) Image-based quantification of MAC-2-positive stained cells. (WT, ; TLR9 KO, ; SERCA2a KO, ; and SERCA2a/TLR9 KO, ). (c) Plasma levels of interleukin-6 (IL-6) and (d) tumor necrosis factor (TNF) determined by Luminex assays (WT, ; TLR9 KO, ; SERCA2a KO, ; and SERCA2a/TLR9 KO, ). (e) Left ventricular gene expression of IL-6 and (f) TNF determined by real-time RT-PCR and presented relative to the gene expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (WT, ; TLR9 KO, ; SERCA2a KO, ; and SERCA2a/TLR9 KO, ). The groups were compared using two-way ANOVA with Tukey’s multiple comparison test post hoc. Data are mean ± SEM. versus corresponding KO control.

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