Neuroinflammation as Fuel for Axonal Regeneration in the Injured Vertebrate Central Nervous System

Figure 2

Intravitreal injection of zymosan accelerates axonal regeneration in zebrafish. (a) Representative images of biocytin-labeled axons in the contralateral optic tectum of naive fish (left) and fish treated with vehicle or zymosan, at 7 days after optic nerve injury (middle and right, resp.). The stratum opticum (SO), the layer through which the RGC axons innervate the tectum, is indicated. Scale bar = 50 µm. (b) Quantification of the reinnervated area of the optic tectum in fish treated with vehicle or zymosan at 7 days after injury, relative to naive fish. Intravitreal injection of zymosan significantly accelerates reinnervation, which is already close to naive levels in zymosan treated fish as compared to vehicle-injected fish. Data represent mean ± SEM ( animals per group, , and ).

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