Middermal Elastolysis: Dermal Fibroblasts Cooperate with Inflammatory Cells to the Elastolytic Disorder

Figure 3

Skin biopsies from MDE patient (b, d, f, and h) and healthy donor (a, c, e, and g). M1 macrophages (iNOS positive) are present in MDE areas together with iNOS negative macrophages (b). No reaction is noticed in the middermis of control patients (a). A weak reaction for MMP-2 (c) and TIMP-2 (e) is detected on cell membranes in the middermis of a control subject. A positive reaction for MMP-2 (d) and TIMP-2 (f) is evident on the cell surface of middermal fibroblasts and inflammatory cells. A diffuse reaction for MMP-14 is localized in large areas of the middermis from MDE patient (h). A mild reaction for MMP-14 is present also on cell membranes in the middermis from a control subject (g). (a-b) Original magnification ×100; (c-d) original magnification ×200; (e-f) original magnification ×100; original magnification ×40.

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