Effects of NSAIDs on the Release of Calcitonin Gene-Related Peptide and Prostaglandin E₂ from Rat Trigeminal Ganglia

Figure 1

(a) Effect of oral administration of 10 mg/Kg nimesulide, ketoprofen, etoricoxib, or water on CGRP release from trigeminal ganglia after in vitro stimulation with inflammatory soup (IS). Thirty minutes and 2 hours after administration, rats were sacrificed, trigeminal ganglia were collected and maintained in vitro with DMEM alone for 5 minutes, then medium was collected, and ganglia were stimulated with IS for another 5 minutes. CGRP released in media basally and upon stimulation was measured by EIA. CGRP levels are expressed as % of basal values (measured before addition of IS) that were normalised to 100. Values are mean ± SEM of 6 animals. ; versus basal; °; °°; °°° versus water + IS. (b) Dose-dependent inhibition of CGRP release in vitro by trigeminal ganglia after stimulation with IS by nimesulide, ketoprofen, and etoricoxib. Rats were treated orally with NSAIDs at the doses of 10, 5, and 1 mg/Kg. Two hours after administration, rats were sacrificed, and experiment was carried on as in (a). Values are mean ± SEM of 6 animals. ; versus basal; °; °°° versus water + IS.

(a)

(b)