Effects of NSAIDs on the Release of Calcitonin Gene-Related Peptide and Prostaglandin E₂ from Rat Trigeminal Ganglia

Figure 2

(a) Effect of oral administration of 10 mg/Kg nimesulide, ketoprofen, etoricoxib, or water on PGE₂ release by trigeminal ganglia both in basal condition and after in vitro stimulation with inflammatory soup (IS). Thirty minutes and 2 hours after administration, rats were sacrificed, trigeminal ganglia were collected and maintained in vitro with DMEM alone for 5 minutes, then medium was collected, and ganglia were stimulated with inflammatory soup for another 5 minutes. Values are mean ± SEM of 6 animals; versus water; °°; °°° versus water + IS. (b) Dose-response curve of the inhibition by nimesulide, ketoprofen, and etoricoxib treatments on in vitro basal and stimulated PGE₂ releases by trigeminal ganglia. Rats were treated orally with NSAIDs at the doses of 10, 5, and 1 mg/Kg. Two hours after administration, rats were sacrificed, trigeminal ganglia were collected and treated as in (a). PGE₂ release was measured in medium by EIA method. Values are mean ± SEM of 6 animals , versus water; °°; °°° versus water + IS.

(a)

(b)