MicroRNA-146a Alleviates Experimental Autoimmune Anterior Uveitis in the Eyes of Lewis Rats

Figure 6

Effect of miR-146a on DNA-binding activity of NF-κB by EMSA. The DNA-binding activity of NF-κB was maximal in lane 4 (MAA group) and lane 5 (MAA + negative mimic group). Following treatment with miR-146a, NF-κB/DNA binding activity was sequentially reduced (lanes 6 and 7). Lane 8 (100-fold unlabeled NF-κB probe) confirmed these changes as NF-κB specific. Ctrl = control; MAA = melanin-associated antigen; miR-146a L = low-dose miR-146a; miR-146 H = high-dose miR146a.