Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

<div>CPS antibody binds to M. ovipneumoniae in vitro. 1 × 1010 CFU (colony formation units) of M. ovipneumoniae solution were prepared, and the CFU value was adjusted by dilution before the test was conducted. For optimization of the immune reaction, 1 × 105 CFU were investigated. The bacterial cells were incubated with rabbit anti-M. ovipneumoniae CPS IgG (1 : 100) and rabbit nonspecific IgG and then probed with Rhodamine- (TRITC-) labeled secondary antibody for red fluorescence. Representative images of confocal microscopy for M. ovipneumoniae after binding of CPS antibody indicate the presence of CPS (red) and confirm the validity of CPS antibody. Bars in (a)–(d): 100 μm.</div>

Mediators of Inflammation

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Figure 1

Figure 1: Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Figure 1 | Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells 