Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Figure 3

The impact of CPS on the expression of MyD88-dependent TLR signaling pathway in ALI cultures of sheep bronchial epithelial cells. 4-week old ALI cultures of sheep bronchial epithelial cells were exposed to indicated conditions for 48 h. (a) The expression of several key components of MyD88-dependent signaling cascade, including the MyD88, IRAK1, IRAK2, IRAK4, TRAF6, and TAB1 transcripts were determined by qRT-PCR assays. In comparison with the controls, all tested transcripts showed a significant increase in the cells treated with CPS or M. ovipneumoniae. (b) Immunoblotting assay also showed an evoked expression of all MyD88-dependent signaling-associated proteins in ALI cultures upon CPS/MO. ovipneumoniae stimulation. (c) Densitometric analysis of western blot showed MyD88, IRAK1, IRAK2, IRAK4, TRAF6, and TAB1 expression over β-actin. Values are mean ± SD for at least three independent experiments performed in triplicate. versus that of the control. Compared between indicated groups, ^▲▲.

(a)

(b)

(c)