Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Figure 5

The impact of CPS on the expression of TLR signaling-associated nuclear transcription factors in ALI cultures of sheep bronchial epithelial cells. 4-week old ALI cultures of sheep bronchial epithelial cells were exposed to the indicated conditions for 48 h. (a) The expression of several key nuclear transcription factors, including the IRF5, NF-κB, and IRF3 transcripts, were determined by qRT-PCR assays. An extremely more abundant IRF5 and NF-κB mRNA was detected in ALI cultures treated with CPS/MO, while no evident alternation of IRF3 expression were observed in M. ovipneumoniae-infected cells. (b) Immunoblotting assay also showed the expression of p-NF-κB, p-AP-1 and IRF3 protein, but not the IRF5 protein, was increased in ALI cultures upon CPS/M. ovipneumoniae stimulation. (c) Densitometric values are shown for p-NF-κB, p-AP-1, IRF3, and IRF5 proteins over β-actin. These bar graphs shown here are representative of three independent experiments with similar results. and versus that of the control. Compared between indicated groups, ^▲ and ^▲▲.

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