Influence of TNF and IL17 Gene Polymorphisms on the Spondyloarthritis Immunopathogenesis, Regardless of HLA-B27, in a Brazilian Population

Table 1

Primer sequences, conditions, restriction enzymes, and size fragments.

TNF and IL17 SNPs	Primer sequences	Conditions	Restriction enzymes	Size fragments
TNF-238 (rs361525)	5-ATCTGGAGGAAGCGGTAGTG-3; 5-AGAAGACCCCCCTCGGAACC-3	94°C—5 min	MspI	107 pb
				87 + 20 pb
		72°C—7 min
		4°C—∞
		TNF-308 (rs1800629)	5-AGGCAATAGGTTTTGAGGGGCCAT-3; 5-TCCTCCCTGCTCCGATTCCG-3	94°C—5 min	NcoI	152 pb
				133 + 19 pb
72°C—7 min
4°C—∞
IL17A-G197A (rs2275913)	5-AACAAGTAAGAATGAAAAGAGGACATGGT-3; 5-CCCCCAATGAGGTCATAGAAGAATC-3	95°C—5 min	EcoNI	68 + 34 pb
				102 + 68 + 34 pb
				102 pb
		72°C—10 min
		4°C—∞
IL17F-T7488C (rs763780)	5-ACCAAGGCTGCTCTGTTTCT-3; 5-GGTAAGGAGTGGCATTTCTA-3	96°C—5 min	NlaIII	63 + 80 pb
				143 + 80 + 63 pb
				143 pb
		72°C—7 min
		4°C—∞