Neutrophils Release Metalloproteinases during Adhesion in the Presence of Insulin, but Cathepsin G in the Presence of Glucagon

Figure 2

Effect of hormones on the amino acid composition of proteins secreted by neutrophils in adhesion to fibronectin. Human neutrophils were attached to a substrate coated with fibronectin for 20 min under control conditions or in the presence of 0.1 μM E2, insulin, or glucagon. The proteins were extracted from the extracellular medium, concentrated, and after acid hydrolysis subjected to amino acid analysis. (a) The amount of detected amino acids in the hydrolysate of proteins secreted by control neutrophils (mean ± SEM) is presented. (b) Comparison of the percentage of individual amino acids in hydrolysates of proteins secreted by neutrophils under control conditions and in the presence of hormones. Amino acid profiles were obtained by summing the results of three independent experiments.

(a)

(b)