Neutrophils Release Metalloproteinases during Adhesion in the Presence of Insulin, but Cathepsin G in the Presence of Glucagon

Table 2

List of proteins secreted by neutrophils in adherence to fibronectin in the presence of hormones.

	Protein name				Peptides matched/total	Coverage, %	MOWSE score
	Control	Insulin	E2	Glucagon
MMP-9_HUMAN		MMP-9	+		18/85	25	240
MMP-8_HUMAN		MMP-8			15/85	31	240
CATG_HUMAN				Cath. G	15/77	40	75
PERM_HUMAN	+	MPO			17/85	21	119
TRFL_HUMAN	+	LF	+	+	29/85	43	240
ALBU_HUMAN	+		Albumin		15/28	24	130
NGAL_HUMAN	+	NGAL	+		7/15	42	105
LYSC_HUMAN	+	Lysozyme^∗	+			31	98
S10A9_HUMAN	+	S100-A9	+	+	9/19	48	147
S10A8_HUMAN	+		S100-A8	+	5/30	44	84

Neutrophils were attached to fibronectin for 20 minutes under control conditions (marked +), or in the presence of 0.1 μM insulin, 0.1 μM E2, or 0.1 μg glucagon. Mass spectrometric analysis data were taken from experiments with insulin. Analogous proteins that were identified in control experiments or in experiments with E2 or glucagon are marked (+). Mass spectrometric data for cathepsin G are taken from experiments with glucagon, and those for albumin and S100A8 from experiments with E2. Proteins were separated by SDS-PAGE and identified by mass spectrometric analysis. Protein was identified by MSMS analysis. Proteins identified in two or three analogous experiments were included in the list.