Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells

Figure 2

Upregulation and knockdown of SelS in HUVECs. HUVECs with pcDNA3.1-SelS plasmid transfection confirmed by RT-qPCR (a) and by the western blot (b). The mRNA levels (c) and protein expression (d) of SelS determined following the transfection with SelS siRNAs or negative siRNA in HUVECs. The cells were transfected with pcDNA3.1-SelS recombinant plasmid or SelS siRNAs for 30 h. The results are representative of six independent experiments and are presented as mean ± SD (). versus empty vector; versus negative siRNA. E-vector: empty vector; Pc-SelS: pcDNA3.1-SelS plasmid; Neg.RNA: negative siRNA.

(a)

(b)

(c)

(d)