Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients

Figure 1

Trauma reduces IL-1β secretion and NLRP3 expression in isolated CD14⁺ monocytes. (a) Isolated CD14⁺ cells from peripheral mononuclear blood obtained either from trauma patients (TP, ) on admission or healthy volunteers (HV, ) were incubated and treated as described in Patients and Methods. IL-1β secretion (pg/mL) after stimulation with lipopolysaccharide (LPS) was determined by ELISA. (b) Gene expression analysis of NLRP3 as fold change to unstimulated CD14⁺ monocytes which were isolated and treated with LPS as described. Data are shown as mean ± SEM. versus indicated.

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