The TGF-β1 Signaling Pathway as an Attractive Target in the Fibrosis Pathogenesis of Sjögren’s Syndrome

Figure 7

Effects of SB-431542 treatment on the expression of E-cadherin, vimentin, and collagen type by RT-PCR and Western blot. (a, b) E-cadherin, vimentin, and collagen type I gene expression was quantified by semiquantitative RT-PCR in healthy SGEC treated with TGF-β1 + SB-431542 (10 μM) or TGF-β1 (10 ng/ml) alone as control. Band intensities were analyzed by densitometric analysis performed by gel image software, normalized against GAPDH, and expressed in arbitrary units (b). (c, d) Effects of SB-431542 (10 μM) addition on the expression of E-cadherin, vimentin, and collagen type I in TGF-β1-treated healthy SGEC by Western blot. axis of (d) represents the banding densities of samples tested versus β-actin. Data are expressed as a significant change relative to TGF-β1-treated healthy SGEC. Each bar represents ( and ); each experiment was repeated three times.

(a)

(b)

(c)

(d)