Complement C5a Alters the Membrane Potential of Neutrophils during Hemorrhagic Shock

Figure 1

Calibration curves for alterations in the mean fluorescence depending on the calculated membrane potential. Shown here are the linear regression curves for two (solid line) or five different potassium concentrations (dotted line). 4.5 mM potassium was considered the physiological extracellular concentration and set to 100%. The method allows the detection of small alterations in membrane potential with an increase in the fluorescence of 6.0% (solid line) or 5.7% (dotted line) per mV increase in membrane potential. per concentration.