Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells

Figure 2

ADAM17 siRNA attenuated Ang II-induced inflammation in vascular smooth muscle cells. After being transfected with 100 nmol/l ADAM17 siRNA for 30 hours, vascular smooth muscle cells were then stimulated with 100 nmol/l Ang II for 24 hours. (a) ADAM17 protein level, independent experiments. (b) ADAM 17 mRNA level, independent experiments. (c–e) At the end of the experiment, the medium was collected to detect the concentration of IL-1β, IL-6, and TNF-α by ELISA, –5 independent experiments. (c) IL-1β protein level. (d) IL-6 protein level. (e) TNF-α protein level. (f) The mRNA levels of IL-1β, IL-6, and TNF-α, independent experiments. Ang II: angiotensin II; NC: negative control. versus control, versus control, and versus Ang II.

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