Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells

Figure 6

ADAM17 siRNA decreased Ang II-induced activation of EGFR and ERK1/2 in vascular smooth muscle cells. After being transfected with 100 nmol/l ADAM17 siRNA for 30 hours, vascular smooth muscle cells were then stimulated with 100 nmol/l Ang II for 24 hours. (a) EGFR activity, independent experiments; (b) ERK1/2 activity, independent experiments; (c) proposed model of ADAM17 in regulating the inhibitory effect of CGRP on Ang II-induced inflammation through the EGFR-ERK1/2 pathway. Ang II: angiotensin II; NC: negative control; +: positive effect; −: negative effect; VSMCs: vascular smooth muscle cells. versus control, versus control, and versus Ang II.

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