Bisdemethoxycurcumin and Its Cyclized Pyrazole Analogue Differentially Disrupt Lipopolysaccharide Signalling in Human Monocyte-Derived Macrophages

Figure 1

Effects of GG9, dexamethasone (dexa), and CLI-095 on LPS-induced activation of M1 macrophage markers CD80 and CCR2. Cells were pretreated with dexa, GG9, and CLI-095 for 30 min and then polarized using either 0.1 μM LPS (M1) or 20 ng/ml IL-4 plus 5 ng/ml IL-13 (M2) for 24 h in RPMI + 10% FBS as described in Materials and Methods. (a, c) Representative flow cytometry assays for CD80⁺ and CCR2⁺ cells. Bar graphs represent the mean (±SEM) of CD80⁺ (b) and CCR2⁺ (d) mean fluorescence intensity (MFI) from 6 independent experiments. versus resting; and versus LPS.

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