Characterization of Site-Specific Phosphorylation of NF-κB p65 in Retinal Cells in Response to High Glucose and Cytokine Polarization

Figure 1

Degradation of IκBα in HREC versus Müller cells when cultured in high glucose with cytokine treatments. HREC and Müller cells were cultured under normal-glucose (NG, 5 mM) and high-glucose (HG, 25 mM) conditions followed by TNF-α and IL-1β versus IL-4 treatment for 10 minutes (Müller cells only), 30 minutes, 2 hours, and 24 hours. Protein levels of IκBα in HREC (a) and IκBα in Müller cells (b) were detected by Western blot. Data shown are representative of 5 independent experiments in duplicate and are expressed as . versus NG and versus HG.

(a)

(b)