Characterization of Site-Specific Phosphorylation of NF-κB p65 in Retinal Cells in Response to High Glucose and Cytokine Polarization

Figure 7

Protein levels of select NF-κB-regulated inflammatory mediators in Müller cells exposed to high glucose with cytokine treatments. Müller cells were cultured under normal-glucose (NG, 5 mM) and high-glucose (HG, 25 mM) conditions followed by TNF-α and IL-1β versus IL-4 treatment for 24 hours. Protein levels of IL-1β (a), IL-8 (b), IL-10 (c), TNF-α (d), and COX-2 (e) were detected by ELISA or Western blot. Data shown are representative of 5 independent experiments in duplicate and are expressed as . versus NG and versus HG.

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