Characterization of Site-Specific Phosphorylation of NF-κB p65 in Retinal Cells in Response to High Glucose and Cytokine Polarization

Table 1

Primer sequences.

Genes	Definition	Forward (5 to 3)	Reverse (5 to 3)
ICAM-1	Intercellular adhesion molecule 1	CTTCGTGTCCTGTATGGCCC	CACATTGGAGTCTGCTGGGA
VCAM-1	Vascular cell adhesion protein 1	GTCAATGTTGCCCCCAGAGATA	ACAGGATTTTCGGAGCAGGA
IL-1β	Interleukin-1 beta	AGAAGTACCTGAGCTCGCCA	CTGGAAGGAGCACTTCATCTGT
IL-6	Interleukin-6	TACAGGGAGAGGGAGCGATA	CTCAGACATCTCCAGTCCTCT
IL-8	Interleukin-8	AGAGCCAGGAAGAAACCACC	GGCAAAACTGCACCTTCACAC
IL-10	Interleukin-10	AAGACCCAGACATCAAGGCG	AATCGATGACAGCGCCGTAG
TNF-α	Tumor necrosis factor alpha	AGGCGCTCCCCAAGAAGACA	TCCTTGGCAAAACTGCACCT
TNFR	Tumor necrosis factor receptor	CCAGTGCGTTGGACAGAAGG	GAAGAATCTGAGCTCCCGGTG
CXCL-11	C-X-C motif chemokine 11	TTGTTCAAGGCTTCCCCATGT	CCACTTTCACTGCTTTTACCCC
CCL-23	C-C motif chemokine ligand 23	CTGGACATGCTCTGGAGGAGA	GGAGTGAACACGGGATGCTT
COX-2	Cyclooxygenase 2	GCTGTTCCCACCCATGTCAA	AAATTCCGGTGTTGAGCAGT
BAX	Bcl-2-associated X protein	CATGGGCTGGACATTGGACT	GGCAGCCCCCAACCAC
IFNγ	Interferon gamma	TGGAAAGAGGAGAGTGACAGA	ACACTCTTTTGGATGCTCTGGT
IL-17a	Interleukin-17A	CCTTGGAATCTCCACCGCAA	GTGGTAGTCCACGTTCCCAT
iNOS	Inducible nitric oxide synthase	GGACCCTGCAGACAGGC	TTCTTCACTGTGGGGCAAGG
cFLIP	Cellular FLICE-inhibitory protein	CAGCAGGTCTGAGCTTGTCC	AGTGGGGGAGTTGCCCG