Identification of SMAD3 as a Novel Mediator of Inflammation in Human Myometrium In Vitro

Figure 4

Effect of siSMAD3 on poly(I : C)-induced proinflammatory cytokines and chemokines. Human primary myometrial cells, obtained from women at term in the absence of labour, were transfected with or without 50 nM siSMAD3 or 50 nM siCONT for 48 h and then treated with 5 μg/ml poly(I : C) for an additional 24 h ( patients). (a–d) Expression of IL-1α, IL-6, IL-8, and MCP-1 mRNA was analysed by qRT-PCR. (e–g) IL-6, IL-8, and MCP-1 concentrations in the incubation medium were assayed by ELISA. For all data, the fold change was calculated relative to poly(I : C)-stimulated siCONT-transfected cells and displayed as mean ± SEM. vs. poly(I : C)-stimulated siCONT-transfected cells (one-way ANOVA).

(a)

(b)

(c)

(d)

(e)

(f)

(g)