Identification of SMAD3 as a Novel Mediator of Inflammation in Human Myometrium In Vitro
Effect of siSMAD3 on the expression and secretion of adhesion molecules. Human primary myometrial cells, obtained from women at term in the absence of labour, were transfected with or without 50 nM siSMAD3 or 50 nM siCONT for 48 h and then treated with (a–d) 1 ng/ml IL-1β or (e–h) 5 μg/ml poly(I : C) for an additional 24 h ( patients). (a, c, e, g) Expression of ICAM-1 and VCAM-1 mRNA was analysed by qRT-PCR. (b, d, f, h) sICAM-1 and sVCAM-1 concentrations in the incubation media were assayed by ELISA. The fold change was calculated relative to IL-1β- or poly(I : C)-stimulated siCONT-transfected cells and data displayed as mean ± SEM. vs. IL-1β-stimulated siCONT-transfected cells (one-way ANOVA). vs. poly(I : C)-stimulated siCONT-transfected cells (one-way ANOVA).
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