Identification of SMAD3 as a Novel Mediator of Inflammation in Human Myometrium In Vitro
Effect of siSMAD3 on the COX-2-prostaglandin pathway. Human primary myometrial cells, obtained from women at term in the absence of labour, were transfected with or without 50 nM siSMAD3 or 50 nM siCONT for 48 h and then treated with (a–c) 1 ng/ml IL-1β or (d–f) 5 μg/ml poly(I : C) for an additional 24 h ( patients). (a, c, d, e) Expression of COX-2 and FP mRNA was analysed by qRT-PCR. (c, f) PGF2α concentration in the incubation medium was assayed by ELISA. For all data, the fold change was calculated relative to IL-1β- or poly(I : C)-stimulated siCONT-transfected cells and displayed as mean ± SEM. vs. IL-1β-stimulated siCONT-transfected cells (one-way ANOVA). vs. poly(I : C)-stimulated siCONT-transfected cells (one-way ANOVA).
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