Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway

Figure 4

The effect of GSPE and BAY11-7082 on the invasion ability of ECA109 cells. The effect of GSPE on ECA109 invasion was analyzed by using Transwell assay. Each treatment was assayed in triplicate. After incubation at 37°C for 24 h, the cells were observed by an inverted microscope (magnification, ×100). (a–d) The inhibition of invasion ability in cells induced by GSPE (0–80 μg/mL). (e–h) The inhibition of invasion ability in cells induced by GSPE (0–80 μg/mL) + BAY11-7082. (i) The number of invaded cells, which was evaluated by ImageJ 2x. Each column represents mean ± SD of three groups of independent samples. compared with the BAY11-7082 group; compared with the GSPE 0 group.

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