Critical Role of HAX-1 in Promoting Avian Influenza Virus Replication in Lung Epithelial Cells

Figure 2

PB1-F2 interacted with HAX-1. HEK293T cells were transfected with pRK5-Flag-PB1-F2 or pRK5-HA-HAX-1. The NP-40 soluble fraction (S) and insoluble pellet (P) of the cell lysates were subjected to western blot analysis with either anti-Flag or anti-HA antibodies to detect expression of PB1-F2 or HAX-1. (a) Only the soluble fraction was tested for HAX-1 expression. (b) Coimmunoprecipitation of PB1-F2 and HAX-1. HEK293T cells were mock transfected or transfected with pRK5-Flag-PB1-F2 and pRK5-HA-HAX-1. The cell lysates were prepared and incubated with either anti-Flag (left) or anti-HA (right) antibody and immunoprecipitated, which were subjected to western blot analysis with either anti-HA (left) or anti-Flag antibody (right).

(a)

(b)