Mediators of Inflammation / 2018 / Article / Fig 5

Research Article

Placental Ras Regulates Inflammation Associated with Maternal Obesity

Figure 5

Effect of siKRAS and siHRAS on LPS-induced inflammation in human primary trophoblast cells. Human primary trophoblast cells were transfected with 50 nM siKRAS, 50 mM siHRAS, or 50 mM siCONT for 48 h then treated with 1 μg/ml LPS for an additional 24 h ( patients). (a–d) IL-6, IL-8, MCP-1, and GRO-α mRNA expression was analysed by qRT-PCR, and the fold change was calculated relative to LPS-stimulated siCONT-transfected cells. (e–h) The incubation medium was assayed for concentration of IL-6, IL-8, MCP-1, and GRO-α release by ELISA. Data are displayed as mean ± SEM. vs. LPS-stimulated siCONT-transfected cells (repeated measures one-way ANOVA with Fisher’s LSD post-hoc test).
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.