Placental Ras Regulates Inflammation Associated with Maternal Obesity

Figure 8

Effect of siKRAS on LPS-induced ERK1/2 activation. Human primary trophoblast cells were transfected with 50 nM siKRAS or 50 nM siCONT for 48 h then treated with 1 μg/ml LPS for an additional 24 h ( patients). Phosphorylated ERK (p-ERK) protein expression was assessed by Western blot and normalised to total ERK. A representative Western blot from 1 patient is shown. The fold change was calculated relative to LPS. vs. LPS (repeated measures one-way ANOVA with Fisher’s LSD post-hoc test).