Figure 8: Impact of GSK3β signaling in U937 cells in response to H37Rv infection. In the presence or absence of GSK3β inhibitor LiCl, the coculture model of A549/U937 cells was infected with H37Rv mycobacteria from the upper chamber (A549 cells, AI), lower chamber (U937 cells, UI), or both chambers (A549 and U937 cells, CI) at a MOI of 3 for 18 h before the culture medium and U937 cells were harvested for analysis by RT-PCR assay. Inductions of indicated transcripts of U937 cells infected with H37Rv in different conditions. The data was presented as the fold of changes of indicated transcripts over the noninfected cells. An increased abundance of indicated TLR signaling and cytokines was observed in the presence of GSK3β inhibitor LiCl, but the trend of a reduction of TLR-mediated inflammatory responses was not altered by the addition of LiCl. Error bars represent the standard deviation (SD) from three independent experiments. Compared to noninfection (NI) control, and ; compared to the absence of LY294002, and . NI: noninfected control; AI: infection was performed on A549 cell alone; UI: infection was performed on U937 alone; CI: infection was performed on both A549 cells and U937 cells.