A Profibrotic Phenotype in Naïve and in Fibrotic Lung Myofibroblasts Is Governed by Modulations in Thy-1 Expression and Activation

Figure 2

Proliferation of myofibroblasts from WT- and Thy1-deficient (DEF) mice. (a) Cell mass of myofibroblasts isolated from WT and Thy1 (DEF) mice measured after 24 h by methylene blue staining, followed by colorimetric assay. The results are presented as fold change from day 0 ( in each group) (). (b) BrdU uptake in myofibroblasts from WT (Thy1⁺) and Thy1 (DEF) mice (Thy1⁻) previously treated with 10 μM BrdU for 24 h. BrdU uptake was assessed by anti-BrdU-FITC-conjugated mAb staining versus PI-total DNA staining followed by flow cytometry.

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