Anti-Inflammatory Effects of Aster incisus through the Inhibition of NF-κB, MAPK, and Akt Pathways in LPS-Stimulated RAW 264.7 Macrophages

Figure 6

Aster incisus effects on the MAPK pathway. RAW 264.7 macrophages cell were cultured in DMEM, treated with Aster incisus, and activated with LPS, and whole cell lysates were obtained and proteins were quantified before being separated by Western blot. The phosphorylated and nonphosphorylated proteins of the MAPK kinase pathway were analyzed as shown above. (b) Aster incisus effects on the PI3K-Akt pathway. RAW 264.7 macrophage cells were treated with Aster incisus for 24 h and activated with LPS for 30 min, followed by protein electrophoresis. These cells were viewed by Western blot. The phosphorylated and nonphosphorylated proteins of the Akt pathway were analyzed using specific antibodies, and GAPDH was used as a standard protein. Differences between treatment groups and the LPS-stimulated nontreated group were considered significant at a value of . The statistical difference among the control and the LPS-stimulated nontreated groups was significant at a value of .

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