Neuropeptides SP and CGRP Diminish the Moraxella catarrhalis Outer Membrane Vesicle- (OMV-) Triggered Inflammatory Response of Human A549 Epithelial Cells and Neutrophils
Table 1
Percentage of live, apoptotic, and dead A549 cells expressed as and analyzed by Annexin V and Dead Cell assay kit using Muse Cell Analyzer.
Live
Total apoptotic
Dead
8 h of incubation
Untreated control
88.42 ± 1.45
10.72 ± 0.99
0.87 ± 0.29
Positive control H2O2 (5 mM)
67.07 ± 2.60
27.98 ± 4.84
4.93 ± 2.30
OMVs (20 μg/ml)
82.21 ± 0.35
17.70 ± 0.28
0.28 ± 0.18
OMVs (20 μg/ml) + SP (10−8 M)
81.64 ± 5.56
17.83 ± 5.06
0.5 ± 0.49
OMVs (20 μg/ml) + CGRP (10−8 M)
84.78 ± 0.81
14.90 ± 0.78
0.23 ± 0.18
OMVs (100 μg/ml)
78.28 ± 1.31
20.23 ± 3.01
0.38 ± 0.39
OMVs (100 μg/ml) + SP (10−8 M)
82.18 ± 1.45
17.40 ± 1.48
0.43 ± 0.04
OMVs (100 μg/ml) + CGRP (10−8 M)
86.00 ± 2.33
13.40 ± 2.19
0.6 ± 0.14
SP (10−8 M)
ND
ND
ND
CGRP (10−8 M)
ND
ND
ND
24 h of incubation
Untreated control
86.25 ± 2.14
12.88 ± 1.8
0.87 ± 0.55
Positive control H2O2 (5 mM)
38.51 ± 0.35
59.99 ± 0.98
1.49 ± 1.34
OMVs (20 μg/ml)
83.98 ± 2.86
15.00 ± 3.04
1.03 ± 0.18
OMVs (20 μg/ml) + SP (10−8 M)
90.08 ± 0.60
9.10 ± 0.21
0.83 ± 0.39
OMVs (20 μg/ml) + CGRP (10−8 M)
83.33 ± 2.72
15.83 ± 2.72
0.85 ± 0.01
OMVs (100 μg/ml)
68.25 ± 2.18
30.98 ± 2.20
0.70 ± 0.13
OMVs (100 μg/ml) + SP (10−8 M)
80.05 ± 0.07
17.80 ± 1.98
2.15 ± 2.05
OMVs (100 μg/ml) + CGRP (10−8 M)
83.68 ± 3.08
14.85 ± 1.34
1.48 ± 1.73
SP (10−8 M)
82.23 ± 0.46
15.30 ± 1.70
2.48 ± 2.16
CGRP (10−8 M)
89.33 ± 1.52
9.43 ± 2.05
1.23 ± 0.53
Total apoptotic refers to early apoptotic and late apoptotic cells. ND: not determined.
