Osthole Protects against Acute Lung Injury by Suppressing NF-κB-Dependent Inflammation

Figure 1

Osthole inhibits LPS-induced inflammatory cytokines in mouse peritoneal macrophages (MPMs). (a) The chemical structure of osthole. (b) MPMs were treated with various concentrations of osthole for 24 h. The viability of cells was measured by MTT assay and values reported as ; . (c) MPMs were treated with osthole (20, 50, or 100 μM) for 24 h, and light micrographs were recorded using a microscope. Representative of three evaluations, , micron . (d–e) MPMs were pretreated with osthole (20, 50, or 100 μM) for 0.5 h and stimulated with LPS (0.5 μg/mL) for 24 h, and secreted IL-6 (d) and TNF-α (e) were measured from conditioned media by ELISA (Materials and Methods). Values normalized to total protein of cells and reported as relative to LPS alone; . (f–g) MPMs were pretreated with osthole (20, 50, or 100 μM) for 0.5 h and stimulated with LPS (0.5 μg/mL) for 6 h, and total RNA was extracted for real-time qPCR for IL-6 (f) and TNF-α (g). Values normalized to β-actin mRNA and reported as ; . (d–g) versus control; versus LPS alone.

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