Research Article

Osthole Protects against Acute Lung Injury by Suppressing NF-κB-Dependent Inflammation

Figure 4

Osthole protects against inflammatory tissue injury in LPS-induced ALI. C57BL/6 mice were pretreated for 0.5 h with 20 mg/kg or 40 mg/kg osthole via ip injection, followed by intratracheal injection of 5 mg/kg LPS, and lungs were sampled 6 h later for analyses. (a, top panel) Representative light micrograph of lung histological changes stained with H&E, (a, middle panel) of immunolocalization of CD68, marker for macrophages (brown), and (a, bottom panel) of tissue localization of TNF-α (brown), micron . Quantification of positive lung tissue staining for (b) CD68 and (c) TNF-α in (a); values are reported as % ; . versus control (Ctrl). versus vehicle.
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