IL-17 Receptor Signaling Negatively Regulates the Development of Tubulointerstitial Fibrosis in the Kidney
Exaggerated TF in Il17ra−/− mice following UUO. WT and Il17ra−/− mice () were subjected to ureteral obstruction. At day 7 post-surgery, UUO kidneys were stained with (a) PAS, (b) Masson’s trichrome, and (c) Picrosirius red stain to evaluate inflammatory changes and TF development. The inflammation score and percentage fibrosis were quantified blindly based on stained sections. Photomicrographs are representative of 2 independent experiments. Original magnification: 4x (a), 20x (b), and 10x (c). (e) The absolute collagen content in the UUO and non-UUO kidneys (–5) was quantified using hydroxyproline assay. (e) Kidneys were evaluated for Il17rc mRNA expression by qPCR. In the scattered plots, each dot represents individual mouse and error bars indicate . The data are pooled from two independent experiments. value ≤0.05 (), ≤0.01 (), ≤0.001 (), and ≤0.0001 ().
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