Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model

Table 1

Identification of immune cells in each panel based upon expression of cell surface markers.

Antibody	Cell population
Panel 1—dendritic cell stain
MHCII^+CD11c^+CD11b^+	CD11b + dendritic cells (proinflammatory) (CD11b^+DCs)
MHCII^+CD11c^+CD103^+	CD103^+ dendritic cells (anti-inflammatory) (CD103^+ DCs)
Panel 2—macrophage/mast cell stain
F4/80^+	Macrophages (Mφ)
IgE^+CD117^+	Mast cells (MC)
Panel 3—T cell and B cell stain
CD3^+CD4^+	T helper cells
CD3^+CD8^+	Cytotoxic T cells
CD19^+	B cells
Panel 4—regulatory T cell stain
CD4^+CD25^+Foxp3^+	Regulatory T cells (Tregs)
Panel 5—neutrophil/natural killer cell/monocyte stain
CD335^+	Natural killer cells (NKs)
Ly6C^+	Monocytes (Mo)
Ly6G^+	Neutrophils (NPh)

The different subsets of leukocytes that were identified during the flow cytometry experiments based upon the expression of cell surface markers and their abbreviations as used in this manuscript between round brackets.