Inflammation and LPS-Binding Protein Enable the Stimulatory Effect of Endotoxin on Prolactin Secretion in the Ovine Anterior Pituitary: Ex Vivo Study

Table 2

The effects LPS, GnRH, and LBP on the relative gene expression of PRL in the AP explants.

Gene	Animals	Anterior pituitary explants
		Control	GnRH	LPS	LPS + GnRH	LPS + LBP	LPS + LBP + GnRH
PRL	Saline-treated	1 ± 0.1^A	2.5 ± 0.2^BC	1.3 ± 0.3 ^AB	2.9 ± 0.3^C	2.8 ± 0.1^C	5.6 ± 0.3^D
	LPS-treated	4.1 ± 0.4^A	5.7 ± 0.2^B	8.2 ± 0.3^C	10.1 ± 0.2^D	7.4 ± 0.4 ^C	9.9 ± 0.8 ^D

All data are presented as the mean (± S.E.M.). Different capital letters indicate significant () differences according to repeated-measures three-way analysis of variance (ANOVA, GraphPad Prism, San Diego, CA, USA) followed by a post hoc Sidak’s multiple comparison test. Gene expression data were presented as normalized to the control of saline-treated group.