Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress

Table 1

The primer sequences for qPCR analysis.

Gene		Sequence of primers (5-3)	Amplicon Size (bp)
Sema3A	Forward	CAGCCATGTACAACCCAGTG	154
	Reverse	ACGGTTCCAACATCTGTTCC
NRP-1	Forward	GGAGCTACTGGGCTGTGAAG	187
	Reverse	ACCGTATGTCGGGAACTCTG
PLXA1	Forward	GTGTGTGGATAGCCATCA	433
	Reverse	CCAGCCTCTCGAACACT
IL-lβ	Forward	TGTGCAAGTGTCTGAAGCAGCTATG	1001
	Reverse	ACACAGGCTCTCTTTGAACAGAATG
TNF-α	Forward	CTCCCTCCAGAAAAGACACCATGA	982
	Reverse	CTGACCACTCTCCCTTTGCAGAAC
COX-2	Forward	CAGTTTCTCTACAACAACTCCATC	161
	Reverse	TTCATCTCTCTGCTCTGGTC
MMP-3	Forward	CTCAAGGGTGGATGCTGTC	234
	Reverse	TGCCATAGCACATGCTGAAC
MMP-13	Forward	CC AAAAGAGGT GAAGAGACTGA	128
	Reverse	CGGGGATAATCTTTGTCCATA
Rnd1	Forward	CATCAGCCGTCCAGAGACCA	111
	Reverse	CGAAGATCTGTCTTGCAGCCAATA
GAPDH	Forward	ATCATCCCTGCATCCACT	156
	Reverse	GTCATCATACTTGGCAGGTTTC