Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34⁺ Cells

Figure 1

Survival and phenotype of CD34⁺ cells from CB or mPB in the presence of combined proinflammatory cytokines. (a) Percentage of live CD34⁺ cells from CB (indicated as negative for Annexin V and PI (black columns, ) or mPB (grey columns, ) in vitro treated for 24 hours with a two-by-two-factor combination and assessed using Annexin V/PI staining, as described in Methods. (b) Percentage of live CD34⁺ cells in the presence of multiple combinations of proinflammatory cytokines. (c–d) Box-plot graphs with fold change of gMFI for CD13 and CD44 expression in CD34⁺ cells after treatment with different combinations of inflammatory cytokines. Dot lines were used to mark control samples without any treatment. All data are presented as of (as above described) experiments performed in duplicate (, , and versus untreated cells (control)). ( CB versus mPB).

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