Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34⁺ Cells

Figure 2

Clonogenic output of CB- and mPB-derived CD34⁺ cells after combined inflammatory stimuli treatment. Comparison of CFU-C formation between CB- ( independent experiments) and mPB-derived ( independent experiments) CD34⁺ cells cultured for 14 days in methylcellulose-based medium is shown. The total CFU-C output was assessed in the presence of inflammatory cytokines with the two-by-two combination (a) or multiple combinations (b). The CFU-GM (c–d) and BFU-E (e–f) output was assessed. The results are expressed as growth fold change of inflammatory cytokine-treated versus untreated cells (control). Control samples were marked with a dot line. All data are presented as (, versus untreated cells (control)). ( CB versus mPB).

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