Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34⁺ Cells

Figure 3

Migratory response of CB- and mPB-derived CD34⁺ cells in the presence of combined inflammatory stimuli. (a) The absolute number of circulating CD34⁺ cells from CB unit () or mPB () and coexpressing the CXCR4 receptor are shown. (b) Representative histogram of CXCR4 expression in CB- and mPB-derived CD34⁺ cells compared to isotype control. (c) Geometric mean value of CXCR4-positive cells on the CD34⁺ population after isolation from CB () and mPB () units. (d) Migration assay using transwell after o/n spontaneous migration (control) or exposure to CXCL12 (120 ng/mL) or to two-by-two inflammatory cytokines plus CXCL12 as chemoattractants. Percentages of migrated CD34⁺ cells from CB (black columns, ) or mPB (grey columns, ) are shown. (e) Migration assay using transwell after o/n spontaneous migration (control) or exposure to CXCL12 (120 ng/mL) or to CXCL12 plus multiple inflammatory cytokines as chemoattractants. Percentages of migrated CD34⁺ cells from CB (black columns, ) or mPB (grey columns, ) are shown. Data are presented as of (as above described) independent experiments ( and versus untreated cells (control) or CXCL12 alone) ( and CB versus mPB).

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