Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34⁺ Cells

Figure 4

Clonogenic output of CB- or mPB-derived CD34⁺ cells after migration toward different combinations of inflammatory cytokines plus CXCL12. Panels a and b show the fold change of clonogenic potential of CB-derived ( independent experiments) and mPB-derived CD34⁺ cells ( independent experiments) after migration toward CXCL12 with or without the two-by-two combination (a) or multiple (b) combinations of proinflammatory factors (postmigration CFU-C). (c–d) Fold change of CFU-GM (c–d) and BFU-E (e–f) growth after migration towards CXCL12 in the presence of various combinations of cytokines. Dot lines were used to mark control samples after migration towards CXCL12. Results are expressed as mean fold change of (, , and ; versus control cells (CXCL12)) ( and CB versus mPB).

(a)

(b)

(c)

(d)

(e)

(f)