Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation

Figure 2

Effect of APO on inflammasome components. LPS-primed BMDMs (a–d, f) and nonprimed BMDMs (e, g) were pretreated with APO or zVAD (20 μM) and stimulated with (a) 150 μg/mL silica for 3 h, (b) 10 μM nigericin for 1 h, (c) 5 mM ATP for 1 h, (d-e) 2 μg/mL poly (dA:dT) for 1 h, and (f-g) 1.5 μg/mL flagellin for 3 h. Mature IL-1β and caspase-1 cleavage were measured in supernatant (Sup). NLRP3, pro-IL-1β, procaspase-1, and ASC were measured in whole cell extract (WCE) by immunoblotting. β-Actin was used as an internal control. LPS, lipopolysaccharide; BMDMs, bone marrow-derived macrophages; APO, A. princeps extract; IL, interleukin; Nig, nigericin.

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