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Figure 4: Decreased expression of DJ-1 and NDUFS3 in ORN-treated rat testes and sperm. (a) Western blotting detection of DJ-1 protein expression in rat testes. (b) Expression of DJ-1 was normalized against that of β-actin. Note that there was a reduction in DJ-1 in the testes from ORN-treated rats. (c) Western blotting detection of NDUFS3 protein expression in rat testes. (d) Expression of NDUFS3 was normalized against that of β-actin. These results showed that the expression of NDUFS3 decreased significantly in the testes of ORN-treated rats compared to that in control subjects. (e) Western blotting detection of DJ-1 protein expression in rat sperm. (f) Expression of DJ-1 was normalized against that of β-actin. These results showed that the expression of DJ-1 decreased significantly in the sperm of ORN-treated rats compared to that in control subjects. , , one-way ANOVA test, rats in each group.