Research Article
Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death
Figure 2
Expression levels of cytokines and iNOS mRNA by macrophages infected with L. (L.) amazonensis or treated with hydroalcoholic extract of C. brasiliense. (a) L.L.a: L. (L.) amazonensis; C.b.: C. brasiliense. L.L.a + C.b.: peritoneal macrophages from BALB/c mice were infected with promastigotes of L. (L.) amazonensis (10 parasites : 1 macrophage) for 3 h and then treated with hydroalcoholic extract of C. brasiliense (30 μg/ml) for 3 h; L.L.a.: macrophages infected and untreated; control: macrophages uninfected and untreated were incubated only with RPMI during 6 h (negative control) significantly compared with negative control; compared to infected macrophages. (b) In 1.5% agarose gel electrophoresis, the products of reverse transcriptase-polymerase chain reaction for conditions above were revealed with ethidium bromide in a transilluminator. M: molecular marker; C−: amplification negative control (H2O); C+: DNA obtained from macrophages stimulated with 5 μg/mL of lipopolysaccharide (LPS); MO: macrophages (negative control, uninfected and untreated); Cb: macrophages treated with C. brasiliense; LLa: macrophages infected with L. (L.) amazonensis.
| (a) |
| (b) |