Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice

Figure 2

Alloxan diabetes attenuates OVA-induced airway inflammation in lung tissue of sensitized mice. Profile of cells in BAL and lung tissue in diabetic allergen-challenged mice. (a) Total cell counts were determined on 1.5 mL BAL, and differential cell counts were assessed by May-Grünwald Giemsa staining. (b–e) Lung sections were stained with hematoxylin and eosin (H&E) for measurement of inflammatory cells around the airways. Data revealed a different extent of cellular infiltration of the peribronchiolar area. Sections were obtained from lungs of nondiabetic saline-challenged mice (b), diabetic saline-challenged mice (c), nondiabetic OVA-challenged mice (d), and diabetic OVA-challenged mice (e). Lungs were removed 24 h after the last challenge. Six animals were assigned to each group. Scale bar 100 μm. (f) Eosinophil count in lung tissue from nondiabetic or diabetic mice challenged with saline or ovalbumin (OVA). The analysis proceeded 24 h after the last OVA challenge. Data were expressed as mean ± SEM (). and versus nondiabetic saline-challenged mice; , and versus nondiabetic OVA-challenged mice.

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