The Effect and Mechanism of TLR9/KLF4 in FFA-Induced Adipocyte Inflammation

Figure 5

High FFA promotes the expression level of inflammation factors by downregulating KLF4. (a) Different concentrations of PA were used to stimulate cells to detect the protein expression levels of TLR9/KLF4; (b) the mRNA expression levels of P65, IL-6, MCP-1, TNF-α, APN, and IL-1β and the release expression levels of IL-6 and MCP-1 under the 20 μM and 200 μM PA stimulation; (c) detecting the mRNA expression levels of P65, IL-6, MCP-1, TNF-α, APN, and IL-1β after 20 μM PA stimulation and si-KLF4; (d) detecting the release expression levels of IL-6, MCP-1, TNF-α, and APN after 20 μM PA stimulation and si-KLF4; (e) detecting the mRNA expression levels of P65, IL-6, MCP-1, TNF-α, APN, and IL-1β after 200 μM PA stimulation and KLF4 transfection; (f) detecting the release expression levels of IL-6, MCP-1, TNF-α, and APN after 200 μM PA stimulation and KLF4 transfection; (g, h) the levels of TG and GLU after 20 μM PA stimulation and si-KLF4. -test, , , ; the difference has a statistical significance.

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